How To Characterize Individual Nanosize Liposomes with Simple Self-Calibrating Fluorescence Microscopy
نویسندگان
چکیده
منابع مشابه
Spectral self-interference fluorescence microscopy
Spontaneous emission of fluorophores located close to a reflecting surface is modified by the interference between direct and reflected waves. The spectral patterns of fluorescent emission near reflecting surfaces can be precisely described with a classical model that considers the relative intensity and polarization state of direct and reflected waves depending on dipole orientation. An algori...
متن کاملHigh resolution spectral self-interference fluorescence microscopy
We present a new method of fluorescence imaging, which yields nm-scale axial height determination and ~15 nm axial resolution. The method uses the unique spectral signature of the fluorescent emission intensity well above a reflecting surface to determine vertical position unambiguously. We have demonstrated axial height determination with nm sensitivity by resolving the height difference of fl...
متن کاملFluorescence Imaging with Nanometer Precision Using Spectral Self-interference Microscopy
Spectral Self-Interference Fluorescence Microscopy (SSFM) has been shown to allow nanometer-scale localization of fluorescent layers placed above a reflecting substrate. A MonteCarlo analysis is used to show how this high localization accuracy can still be expected at the low signal levels associated with single-molecule studies. Discrimination of fluorophores separated by a few tens of nanomet...
متن کاملDetermination of properties of individual liposomes by capillary electrophoresis with postocolumn laser-induced fluorescence detection.
Individual liposome measurements by capillary electrophoresis with postcolumn laser-induced fluorescence detection facilitated the determination of liposome property distributions, two-dimensional plots, and an improved characterization of a liposomal preparation. This advancement in liposome analysis was feasible by using a high-sensitivity postcolumn laser-induced fluorescence detector wired ...
متن کاملStructural characterization of individual vesicles using fluorescence microscopy.
Extrusion of hydrated lipid suspensions is frequently employed to produce vesicles of uniform size, and the resulting vesicles are often reported to be unilamellar. We describe a method for the quantitative fluorescence image analysis of individual vesicles to obtain information on the size, lamellarity, and structure of vesicles produced by extrusion. In contrast to methods for bulk analysis, ...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Nano Letters
سال: 2018
ISSN: 1530-6984,1530-6992
DOI: 10.1021/acs.nanolett.7b05312